"There is no justification for the closure of the Rafah crossing," Hamad al-Raqb, a spokesman for the protesters, said in a speech marking the beginning of a four-day sit-in.
"It is unbecoming of the historic relations between Egyptians and Palestinians," he insisted.
Leading Hamas figures and MPs attended the launch ceremony.
Egyptian authorities have tightened their control over the border with the Hamas-run Gaza Strip since last July's ouster of elected president Mohamed Morsi by the Egyptian army.
In recent weeks, Egypt has repeatedly closed the Rafah crossing, which – due to an ongoing, eight-year Israeli siege – represents Gaza's only window to the outside world.
The Egyptian army has also launched a campaign aimed at destroying the network of tunnels linking Gaza to Egypt's Sinai Peninsula, which in recent years had been used to bring vital commodities into the besieged coastal enclave, home to nearly 2 million Palestinians.
Hamas, an ideological offshoot of the Muslim Brotherhood, the group which propelled Morsi to power in the 2012 elections, has controlled the Gaza Strip, since 2007.
Poland has further fuelled conflict between the two nations as a city took down the brotherhood-in-arms of Polish and Red Army soldiers memorial.
Eurotunnel services disrupted after points failure after 150 migrants try to enter restricted areas of Calais terminal
Iraq's Parliament speaker has urged the Iraqi government to provide secure return to displaced people
Despite the differences between the two countries, Russian-American relations must be based on equal treatment and mutual respect
Spanish police have arrested a Moroccan for distributing ISIL propaganda.
Fierce fighting breaks out between Libyan army, attached to the Government of Tobruk, and other armed groups in the east
Rallies around the country have marked the end of campaigning to approve or reject proposals from Greece's creditors
Germany had reformed its BfV following its failure to prevenet a series of Neo-Nazi murders.
Two people have been killed while walking past mud house in search of accommodation during Thursday night’s rainstorm
The Southern African Development Community have decided to send a team to the Kingdom of Lesotho to investigate the murder of a former military commander
According to IMF data, Greece is followed by Italy, Portugal, Ireland, Greek Cypriot administration and Belgium in a list of countries with high debts compared to their GDP
Canada granted permissions for foreign countries to interrogate their citizens after they were assured that they would not be subject to torture
Brazil has unveiled the new Olympic torch and 12,000 torchbearers will carry flame along 20,000-kilometer route around Brazil
French President Francois Hollande is ready to to hold a summit regarding Boko Haram
A non-governmental organization has raised alarm over an increase in Islamophobic attacks in France six months after the January Charlie Hebdo shootings