"There is no justification for the closure of the Rafah crossing," Hamad al-Raqb, a spokesman for the protesters, said in a speech marking the beginning of a four-day sit-in.
"It is unbecoming of the historic relations between Egyptians and Palestinians," he insisted.
Leading Hamas figures and MPs attended the launch ceremony.
Egyptian authorities have tightened their control over the border with the Hamas-run Gaza Strip since last July's ouster of elected president Mohamed Morsi by the Egyptian army.
In recent weeks, Egypt has repeatedly closed the Rafah crossing, which – due to an ongoing, eight-year Israeli siege – represents Gaza's only window to the outside world.
The Egyptian army has also launched a campaign aimed at destroying the network of tunnels linking Gaza to Egypt's Sinai Peninsula, which in recent years had been used to bring vital commodities into the besieged coastal enclave, home to nearly 2 million Palestinians.
Hamas, an ideological offshoot of the Muslim Brotherhood, the group which propelled Morsi to power in the 2012 elections, has controlled the Gaza Strip, since 2007.
Police said on Twitter that the four youths had been detained for attacking soldiers with fire crackers and causing disturbances.
Merkel's remarks come after EU leaders summit in Brussels to discuss future of 28-nation bloc
The bombing struck outside a hotel in central Mogadishu, gunfire was also heard at the scene of the attack
The list of Peshmergas to pass through to fight against ISIL been delivered both to Turkey and United States. The first units tomorrow to across Turkey's border.
"Israeli forces stationed at the Kisovim military post opened fire on farmlands [in Khan Younis city]," an eyewitness said
Egypt declared a three-month state of emergency in parts of the northern Sinai Peninsula following a deadly attack at a military checkpoint that killed 33 soldiers
The final television debate between Brazil’s presidential candidates was marked by heated exchanges focused on corruption allegations.
Tabare Vazquez is projected to win but a runoff looms against conservative Lacalle Pou
According to the sources, ten militants were killed and a number of others wounded in the airstrikes.
Russia will, carefully and timely, carry out all its responsibilities of delivering natural gas to Europe, Vladimir Putin has said
Turkey's President said that Syrian Kurdish militants have agreed to accept 1,300 Free Syrian Army troops in Kobani.
The German and Dutch foreign ministers have signed a deal on the disputed coastal waters of Ems River
Many then said that Akhmetov - ever the prudent businessman - was cautiously weighing the shifting sands in a volatile situation that had caught him and others by surprise.
Two sheriff's deputies killed in a shooting spree that started in a motel parking lot in Sacramento, authorities said.
Guards opened fire on inmates at the Najayo jail in San Cristobal after the prison was attacked by armed men
It is the strongest measure yet to curb pollution for the Nov. 7-11 meeting in the capital, which has been enveloped by heavy smog over the past few days.