"There is no justification for the closure of the Rafah crossing," Hamad al-Raqb, a spokesman for the protesters, said in a speech marking the beginning of a four-day sit-in.
"It is unbecoming of the historic relations between Egyptians and Palestinians," he insisted.
Leading Hamas figures and MPs attended the launch ceremony.
Egyptian authorities have tightened their control over the border with the Hamas-run Gaza Strip since last July's ouster of elected president Mohamed Morsi by the Egyptian army.
In recent weeks, Egypt has repeatedly closed the Rafah crossing, which – due to an ongoing, eight-year Israeli siege – represents Gaza's only window to the outside world.
The Egyptian army has also launched a campaign aimed at destroying the network of tunnels linking Gaza to Egypt's Sinai Peninsula, which in recent years had been used to bring vital commodities into the besieged coastal enclave, home to nearly 2 million Palestinians.
Hamas, an ideological offshoot of the Muslim Brotherhood, the group which propelled Morsi to power in the 2012 elections, has controlled the Gaza Strip, since 2007.
Palestinian Football Association President Jibril Rajoub has defended his decision to abandon the bid to suspend Israel saying that he has not "suspended the resistance".
A second batch of live anthrax was sent by the US military
The Italian parliament has released the name of a candidate representing southern Italy's cocaine heartland, and is also a candidate for PM Renzi's party.
The US formally removed Cuba from "state sponsored terrorism" list.
Nigerian Finance Minister Akinwumi Adesina became president of the institution on Friday
More than 200 Eritrean refugees have been turned back by border police in the Italian Alps.
Protests have continued in Burundi against the Presidents third bid for Presidency
An ultra orthodox sect in Britain have banned women from driving. Children, whose mothers drive them to their religious school will also be refused entry to the school.
Nigeria's new President has vowed to fight Boko Haram and corruption.
Clashes have caused more than 60,000 to flee the fighting in Mali with escalating violence threatening to derail peace efforts.
The Kyrgyzstan government has banned all unauthorized sermons in certain regions.
The EU and US have pressured Serbia to withrdraw from the Russian-Turkish South Stream project.
Unable to sort their differences, Morocco has begun building a 100km fence on the border with Algeria.
The head of the Council of Europe Thorbjorn Jagland asks Egypt's supreme religious official not to approve over 100 death sentences
Belgium will probe claims of widespread espionage by Germany, which is accused of helping the US spy on Berlin's closest allies in Europe.
The United States will be sending military trainers to Nigeria to help the fight against Boko Haram.