"There is no justification for the closure of the Rafah crossing," Hamad al-Raqb, a spokesman for the protesters, said in a speech marking the beginning of a four-day sit-in.
"It is unbecoming of the historic relations between Egyptians and Palestinians," he insisted.
Leading Hamas figures and MPs attended the launch ceremony.
Egyptian authorities have tightened their control over the border with the Hamas-run Gaza Strip since last July's ouster of elected president Mohamed Morsi by the Egyptian army.
In recent weeks, Egypt has repeatedly closed the Rafah crossing, which – due to an ongoing, eight-year Israeli siege – represents Gaza's only window to the outside world.
The Egyptian army has also launched a campaign aimed at destroying the network of tunnels linking Gaza to Egypt's Sinai Peninsula, which in recent years had been used to bring vital commodities into the besieged coastal enclave, home to nearly 2 million Palestinians.
Hamas, an ideological offshoot of the Muslim Brotherhood, the group which propelled Morsi to power in the 2012 elections, has controlled the Gaza Strip, since 2007.
The use of drones comes at a time when a regular publication of a blacklist of companies using slave labour has been halted.
US President tells African leaders to respect presidential term limits, not to jail journalists, and not to restrict opponents
Child says he was given bag containing explosive by stranger who paid him $5 to bring it to crowded market
US ambassador to Ukraine urges Russia to respect Minsk agreement signed in February
UN Security Council ‘undemocratic, unrepresentative, and exactly where it was in 1945,’ South African govt minister says
A three-year-old child from London is one of hundreds of young people who have been tipped as potential future radicals and extremists.
2,000 in left wing of Syriza call for 'big no' to bailout deal
Russian PM hopes Slovenia will participate in Turkish Stream after talks with his Slovenian counterpart
Russian President adviced European states to rely more on themselves than on military blocks or the U.S.
The annual Trafficking in Persons report has upgraded Cuba, Malaysia, and Uzbekistan to a higher tier as worst offenders for failing to suppress human trafficking.
The ECB will need to extend its money-printing as a result of subdued growth in the Euro zone.
An EU official has said that they "barely know how to talk to ordinary people" and have launched video clips on YouTube to counter misconceptions about the Trans Atlantic partner deal.
Hague-based tribunal attributes move to procedural reasons, but Palestinian diplomat suspects pressure from Israel
Restarting would depend on Syria becoming stable, Russian Union of Gas and Oil Industrialists executive director says.
Viktor Orban suggested that ethnic Hungarians living in Romania should be given an authonomy
Majority in favor of cabinet under Prime Minister Omer Kalyoncu