Turkey's benchmark stock index BIST 100 decreased by 150.79 points or 0.14 percent to open at 109,997.66 points on Wednesday.
The banking sector and holding sector indices fell 0.45 percent and 0.22 percent, respectively.
Among all sector indices, the tourism sector index was the best performer, gaining 0.78 percent, whereas the sports sector index saw the biggest decline with 1.34 percent.
Turkey's benchmark stock index previously closed Tuesday up 1.18 percent to stand at 110,148.45 points with 8 billion Turkish liras ($2.05 billion) trade volume.
Meanwhile, Turkish annual unemployment rate fell to 10.6 percent in August, according to TurkStat. Additionally, Turkish Finance Ministry is set to announce the budget balance.
Separately, global markets will follow the inflation rate in the U.S., which is going to be announced on Wednesday.
The U.S. dollar/Turkish lira exchange rate increased to 3.8930 at 9.30 a.m. local time (0630GMT) Wednesday, compared with 3.8770 at Tuesday's close.
Also, the euro/Turkish lira exchange rate rose to 4.5930 at 9.30 a.m. local time Wednesday, compared with 4.5580 at Tuesday's close.
Brent oil decreased to $61.47 per barrel at 10.30 a.m. local time (0730GMT) compared with $62.42 at Tuesday's close.