"Around 70 activists from the West Bank refugee camps started building Al-Awdah village near Beesan's checkpoint in the northern Jordan Valley," Yasser Abu Kishk of the Popular Committee to Resist Settlement told Anadolu Agency.
"This is a symbolic action for the eventual return of Palestinian refugees to their lands from which they were forced to leave in 1948," Abu Kishk said.
"It’s a stance against the [Israeli] settlement."
Palestinian activists have been establishing symbolic villages by renovating abandoned homes or erecting tents in occupied Palestinian lands, on which Israel plans to build Jewish-only settlements.
The makeshift villages usually stand for a few days before Israeli forces bulldoze them and expel the Palestinians.
An Israeli military force is already besieging the site of Al-Awdah village and banning entry.
On Friday, some 50 activists erected a makeshift village in Ein Hajla region near the Palestinian-Jordanian borders, as a protest against continued Israeli settlement building.
The Jordan Valley is populated by nearly 10,000 Palestinians, who are mostly farmers.
Israel has built nearly 21 Jewish settlements in the area, which it views as an economic and security buffer zone.
Israel wants to keep the region under its control in any future peace deal with the Palestinians.
Palestinians, for their part, insist that the Jordan Valley, which constitutes nearly 30 percent of the occupied West Bank, as part and parcel of the future Palestine.